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  1. TBestDB (View Publication)
    Full Name of the Resource : Taxonomically broad EST database: protist ESTs
    Resource Category : Databases -> Genomic Databases (Non-Human) -> Unicellular Eukaryotes Genome Databases

    Brief Description : The TBestDB (a Taxonomically Broad EST database) database contains ~370,000 clustered EST sequences from 49 organisms, covering a taxonomically broad range of poorly studied, mainly unicellular eukaryotes, and includes experimental information, consensus sequences, gene annotations and metabolic pathway predictions. Most of these ESTs have been generated by the Protist EST Program, a collaboration among six Canadian research groups. EST sequences are read from trace files up to a minimum quality cut-off, vector and linker sequence is masked, and the ESTs are clustered using phrap. The resulting consensus sequences are automatically annotated by the AutoFACT program. The datasets are automatically checked for clustering errors due to chimerism and potential cross-contamination between organisms, and suspect data are flagged in or removed from the database. Access to data deposited in TBestDB by individual users can be restricted to those users for a limited period. With this first report on TBestDB, we open the database to the research community for free processing, annotation, interspecies comparisons and GenBank submission of EST data generated in individual laboratories. For instructions on submission to TBestDB, contact The database can be queried at
    Subject Area : Expressed Sequence Tags

    Institute/s :
    Center Robert-Cedergren for Bioinformatics and Genomics, Departement de Biochimie, Pavillon Roger-Gaudry
    Universite de Montreal, 2900 Edouard-Montpetit, Montreal QC, H3T 1J4, Canada
    Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia B3H 1X5, Canada

    Country : Canada

    Associated Institutes :

    • Département de Biochimie, Canadian Institute for Advanced Research, Robert-Cedergren Centre for Research in Bioinformatics and Genomics, Pavillon Roger-Gaudry, Université de Montréal, 2900 Edouard-Montpetit, Montréal QC Canada
    • Cenix BioScience, Tatzberg 47 01307 Dresden, Germany
    • Department of Biochemistry and Molecular Biology, Dalhousie University Halifax, Nova Scotia, Canada

    Associated Country : Canada

    Authors/Contributors : O'Brien; E.A.; Koski; L.; Zhang; Y.; Yang; L.; Wang E.; Gray M.W.; Burger G.; Lang; B.F.
    Contact Email :
    Year : 2007

    Keywords : Animals; Base Sequence; Cluster Analysis; Consensus Sequence; *Databases, Nucleic Acid; Eukaryota / genetics; Expressed Sequence Tags / *chemistry; Fungi / genetics; Genomics; Internet; User-Computer Interface