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  1. REFOLD (View Publication)
    Full Name of the Resource : Experimental data on protein refolding and purification
    Resource Category : Databases -> Protein Sequence Databases -> Protein properties

    Brief Description : The majority of human proteins expressed in bacteria are insoluble and thus require renaturation. Identifying the optimal refolding conditions and methodology is therefore rate limiting. In order to address this problem, we have catalogued the methods employed in the refolding of proteins in a web-accessible relational database, REFOLD. Entries are heavily annotated such that the database can be searched via multiple parameters. The database will have two roles: First, as a data repository and experimental resource, new data can be rapidly deposited, validated and made available to the refolding and wider scientific community. Much valuable refolding data goes unpublished (for example, in cases where a protein cannot be refolded for structural studies), thus we aim to exploit this untapped resource by encouraging deposition in REFOLD. The database will thus become a powerful experimental tool for the optimization of protein expression, refolding and purification, bypassing the relatively slow and inefficient examination of the literature. Second, REFOLD will become a valuable resource for theoreticians looking for relationships between refolding success and protein characteristics such as charge and hydrophobicity. Data mining may uncover general predictive rules that could facilitate the refolding of novel proteins.
    Subject Area : Protein Refolding; Protein Purification


    Institute/s :
    Department of Biochemistry and Molecular Biology, P.O. Box 13D, Monash University, Vic. 3800, Australia.
    Address of Institute/s :
    Department of Biochemistry and Molecular Biology, P.O. Box 13D, Monash University, Vic. 3800, Australia.
    Country : Australia

    Associated Institutes :

    • Department of Biochemistry and Molecular Biology PO Box 13D Monash University Victoria 3800 Australia
    • Victorian Bioinformatics Consortium PO Box 53 Monash University Clayton, Victoria, 3800, Australia
    • ARC Centre for Structure and Functional Microbial Genomics, Monash University Clayton, Victoria 3800, Australia
    • Swinburne University of Technology Hawthorn Campus, Victoria, 3136, Australia

    Associated Country : Australia


    Authors/Contributors : Buckle, A.M.
    Contact Email : Ashley.Buckle@med.monash.edu.au
    Year : 2006
    Language : English

    Keywords : Databases, Protein; Protein Folding; Recombinant Proteins / isolation & purification / metabolism