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  1. HUGE - Human Unidentified Gene-Encoded large proteins
    Full Name of the Resource : Human unidentified gene-encoded large (>50 kDa) protein and cDNA sequences
    Resource Category : Databases -> Human and Other Vertebrate Genomes -> Human Proteins

    Brief Description : HUGE is a database for human large proteins newly identified in the Kazusa cDNA project, the aim of which is to predict the primary structure of proteins from the sequences of human large cDNAs (>4 kb). In particular, we have been focusing on cDNA clones capable of coding for large proteins (>50 kDa) derived from brain. HUGE contains more than 1600 cDNA sequences with the results of computer analysis of the sequences at DNA and amino acid level, the chromosomal mapping and the expression profile. The risk of containing artifacts in cDNA cloning is considered to be high when cloning long cDNAs. To obtain accurate cDNA sequence data, we examined all of the protein-coding potentiality by GeneMark analysis, and when the warning for coding-interruption was issued, we performed additional experiments using the reverse transcription-coupled polymerase chain reaction method to detect artifacts in cloning, then corrected the sequences. The number of such revision is 220 that comprises 13 % of total cDNA sequences in HUGE. We added the detailed information on this process to HUGE, besides a periodic increase of the number of cDNA entries. HUGE is available through the World Wide Web at

    Institute/s :
    Kazusa DNA Research Institute, Kazusa ‐
    Kamatari, Kisarazu, Chiba, 2920818, Japan

    Address of Institute/s :
    Kazusa DNA Research Institute, Kazusa ‐
    Kamatari, Kisarazu, Chiba, 2920818, Japan

    Country : Japan

    Associated Institutes :

    • Kazusa DNA Research Institute, Kazusa Kamatari, Kisarazu, Chiba, 292 0818, Japan
    • Chiba Industry Advancement Center, Nakase, Mihama ku, Chiba, Japan
    • RIKEN Research Center for Allergy and Immunology, Suehiro, Tsurumi ku, Yokohama, Kanagawa, 230 0045, Japan

    Associated Country : Japan

    Authors/Contributors : Reiko Kikuno; Takahiro Nagase; Manabu Nakayama; Hisashi Koga; Noriko Okazaki; Daisuke Nakajima; Osamu Ohara
    Contact Email :
    Year : 2003
    Language : English